Hs 578t cell

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WebMCF-7 cells, BT-549 cells, Hs-578T, and MCF-10A cells were kindly provided by Prof. Jukka Westermarck (Turku Bioscience, Turku, Finland). 2.2. Enzyme Delivery by Lipofectamine CRISPRMAX Reagent Lipofectamine CRISPRMAX reagent (Invitrogen, Carlsbad, CA, USA) was used for delivering BSA and PKA enzymes in this study. WebHs 578T has been used to measure the calcium-dependent binding of cadherin-11 and N-cadherin. [ 1] It has also been used to study the changes in gene expression induced by …

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WebImmunoprecipitation of periostin protein from Hs 578T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is Periostin (E5F2S) Rabbit mAb. Western blot analysis was performed using Periostin (E5F2S) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as the secondary antibody. WebSix breast cancer cell lines (HS-578T, T47D, MCF-7, BT549, MDA-MB-231, and SKBR-3) were purchased from ... of RFC3 levels with small interfering hsa_circ_0011946 resulted in the inhibition of migration and invasion in MCF-7 cells. Breast cancer patients with high RFC3 levels have a higher risk of distant metastases, 19 thus RFC3 might be an ... recycling coquitlam

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WebHs 578T: Background: It was originated by A.J. Hackett, et al. along with the Hs 578Bst (see ATCC HTB-125), which is a normal fibroblast-like line from the same patient. The Hs 578T cell strain was derived from a carcinoma of the breast. Species: Homo sapiens, human: Tissue: mammary gland/breast: Disease: Carcinoma: Gender: female， 74 years ... Web16 apr. 2024 · HEK293T and Hs 578T cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS). Cells were maintained in a humidified incubator equilibrated with 5% CO 2 at 37°C. MDA-MB-231 cells were cultured in L-15 medium supplemented with 10% FBS and without CO 2. WebHs 578T细胞源自乳房癌，它与正常成纤维细胞样细胞株Hs 578Bst起源于同一位患者。Hs 578T细胞最初是多角形的，但在传代过程中选择并克隆了星形的细胞形态。电镜下观察 … klay thompson retirement

Hs 578T - HTB-126 ATCC

Web8 feb. 2024 · b Flow cytometry indicates that overexpression of SPAG5 inhibit cell apoptosis in MDA-MB-231 and MDA-MB-468 cells, while knockdown of SPAG5 increase cell apoptosis in Hs-578t and BT549 cells. c Western blot of SPAG5, P21, P27, Cyclin A2, Cyclin B1, and CDC20 in MDA-MB-231 and MDA-MB-468 cells treated with SPAG5 … Web1 nov. 2024 · Nonradiolabeled Hs-578T cells pulsed with NSP13-134 peptide or irrelevant HLA-A0301 peptide were used as cold targets. The C:H ratio was 10:1 or 20:1. NSP13-134–specific CTLs were cocultured with hot targets alone or hot targets together with cold targets at a 20:1 E:hot T ratio. recycling corfluteWebBC cell lines BT-474 and BT-20 (cat. nos. 300130 and 300131; CLS Cell Lines Service GmbH), HS-578T (cat. no. 86082104; Sigma-Aldrich; Merck KGaA) and MDA-MB-231 (cat. no. 92024424; Sigma-Aldrich; Merck KGaA) ( 46) were incubated in DMEM/F12 medium (Gibco; Thermo Fisher Scientific, Inc.), supplemented with 5% FBS (Gibco; Thermo … recycling copy machines

"Web12 dec. 2024 · F Cell cycle of Hs-578t cells was analyzed by flow cytometry. G γ-H2AX expression in Hs-578t cells was detected by immunofluorescence 48 h after treatment … " - Hs 578t cell

Hs 578t cell

Antimetastatic Therapies of the Polysulfide Diallyl Trisulfide ... - PLOS

Web2 feb. 2001 · MS-275 at 1 μ m inhibited the proliferation of HS-578t cells without loss of cell viability (>95%viable) as determined by trypan blue exclusion, whereas MS-275 at 1μ m caused a 50% loss of cell viability in MCF-7, ZR-75, and SKBR3 cells after 3 days. Web8 feb. 2024 · Hs-578t and BT549 cells were infected with the lentivirus and selected with puromycin to establish stable SPAG5 knockdown cells. The target sequences of SPAG5 siRNAs, MYCBP shRNAs, and c-MYC siRNAs are listed in (Additional file 1: Table S1). For overexpression, full-length SPAG5 was identified and cloned to generate a Flag-SPAG5 …

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Web9 feb. 2024 · The analysis of polarized cells showed that MDA-MB-231 and Hs 578T cells were highly polarized compared to MCF10A and MCF7 cells at different time points after seeding (Figure 4B). These data might indicate that mesenchymal TNBC cells have a greater migration potential, and the direction of their migration is straightforward from the … WebHS578T. Depmap ID: ACH-000148. CCLE Name: HS578T_BREAST. COSMIC ID: 905957. Sanger ID: 655. Cell Line Passport: SIDM00135. Cellosaurus RRID: CVCL_0332.

Webhs-578t Cells Recommended Transfection Reagents Plasmid DNA transfection and co-transfection: Lipofectamine® 3000 ( >80% transfection efficiency and 3 fold protein … Webtriple negative breast tumor cell lines that share a basal-like morphology. Triple Negative Breast Cancer Panel 2, ... Hs 578T Carcinoma primary CDKN2A homozygous …

WebImmunoprecipitation of periostin protein from Hs 578T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is Periostin (E5F2S) Rabbit mAb. Western blot analysis was performed using Periostin (E5F2S) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as the secondary antibody. Web20 mrt. 2024 · Hs 578T, MDA-MB-468, and MCF-10A cells were initially plated at a density of 5×10 3 cells in 96-well culture plates and cultured for more than 4 h for cell adhesion. Then, cells were treated with ICA of low, medium, and …

WebHs 578T; Breast Carcinoma LGC Standards Buy Hs 578T; Breast Carcinoma online from Cell lines. Certified reference materials for highly accurate and reliable data analysis. We use cookies to ensure that we give you the best experience of our website.

WebCell line name: Hs 578T: Synonyms: HS 578T; Hs-578T; HS-578T; Hs_578t; Hs-578-T; HS-578-T; Hs 578.T; HS578T; Hs578T; Hs578t; HS0578T; 578T; HS578; Hs578; Homo … recycling corby opening times near meWeb7 okt. 2008 · Hep G2 and Hs 578T cells were tested with final concentrations at 250 µg/mL and PC-3 at final concentration of 100 µg/mL. Solutions were added to wells in four replicates. Medium controls and DMSO controls (25 or 10 µL DMSO/mL) were used. Tingenone (250 or 100 µg/mL) was used as a positive control (Setzer et al., 1998). recycling corning caWeb24 mei 2024 · HS‑578T cells also exhibited higher rates of proliferation, invasion and transendothelial migration when compared with MCF‑7 cells. Moreover, it was identified that genetically silencing the COPB2 gene using a lentivirus‑short hairpin RNA inhibited the proliferative, colony formation, migratory and invasive properties of the TNBC HS ... recycling coquitlam bcWebHs 578T Cell - PubChem Apologies, we are having some trouble retrieving data from our servers... PUGVIEW FETCH ERROR: 403 Forbidden National Center for Biotechnology … recycling cornwall ontarioWeb5 jan. 2024 · We used Hs 578T and SUM159PT cell lines, as well as cell clones over-expressing GD3 synthase derived from MDA-MB-231 and MCF-7. Using flow cytometry and immunocytochemistry/confocal microscopy, we report that BC cells express b-series gangliosides GD3 and GD2, as well as significant amounts of O AcGD2. klay thompson shoe brandWeb2.2 Cell Culturing Protocol and Sample Preparation HS 578T breast tumor cells (ATCC) were cultured at 37°C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10 ng/ml epidermal growth factor (EGF), gentamicin, fetal bovine serum at final concentration. 2-3 days. Devices were cleaned recycling corbyWebHs 578T MCF7 MDA-MB-231 MDA-MB-468 T-47D NCI-60 Central Nervous System Cell Lines Shown below are stained trypan blue images and Cellometer counted images of NCI-60 central nervous system cell lines. Counted live cells are outlined in green while the dead trypan blue positive cells are outlined in red. SF-268 SF-295 SF-539 SNB-19 SNB-75 U251 recycling corian countertops